Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy

Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy

Major advances in high-throughput, high-resolution, 3D microscopy techniques have enabled the acquisition of large volumes of neuroanatomical data at submicrometer resolution. One of the first such instruments producing whole-brain-scale data is the Knife-Edge Scanning Microscope (KESM), developed and hosted in the authors' lab. KESM has been used to section and image whole mouse brains at subm...

Knife-Edge Scanning Microscopy: High-throughput Imaging and Analysis of Massive Volumes of Biological Microstructures

Specimen Preparation for Electron Microscopy

In general the SEM sample preparation techniques are more or less similar to the metallographic sample preparation technique for optical microscopy, because both the microscopes reveals the surface topography of sample. In SEM a focussed beam of electrons scans over the specimen surface and the electrons emitted from the sample surface controls the brightness of the CRT spot which also scans th...

Scanning impedance microscopy (SIM): A novel approach for AC transport imaging

Scanning Impedance Microscopy (SIM) is one of the novel scanning probe microscopy (SPM) techniques, which has been developed to taking image from sample surface, providing quantitative information with high lateral resolution on the interface capacitance, and investigating the local capacitance–voltage (C–V) behavior of the interface and AC transport properties. The SIM is an ordinary AFM equip...

Soft-tissue specimen shrinkage during the preparation for scanning electron microscopy

The amount of shrinkage and the scanning electron microscope appearance of specimens of rat tongue, soft palate and small intestine, dehydrated by air­ drying, the critical point technique, the camphene technique and freeze drying were compared. In the specimens of tongue the area shrinkage ranged be­ tween 11,8 and 30,4 per cent, in the soft palate from 19,8 to 37,4 per cent and in the small i...